Recombinant chymosin used for exact and complete removal of a prochymosin derived fusion tag releasing intact native target protein
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چکیده
منابع مشابه
Synthetics of NiFe2O4 nanoparticles for recombinant His-tag protein purification
Recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. Genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. Differed methods for protein purification are categorized, based on cast, quality, Easy work and side effect of protein. In this article, we are investigating his His-tag protein pu...
متن کاملSynthetics of NiFe2O4 nanoparticles for recombinant His-tag protein purification
Recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. Genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. Differed methods for protein purification are categorized, based on cast, quality, Easy work and side effect of protein. In this article, we are investigating his His-tag protein pu...
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Prochymosin (chymosin) contains three disulfide bonds: Cys45-Cys50, Cys206-Cys210, and Cys250-Cys283. We have demonstrated that Cys250-Cys283 is indispensable for correct refolding of prochymosin, whereas Cys45-Cys50 is dispensable but has some contribution to the stability and substrate specificity of the enzyme. Here, we report the results about the functions of Cys206-Cys210 by site-directed...
متن کاملPurification of Tag-Free Recombinant Proteins Using the Profinity eXact Fusion-Tag System affinity purification
Introduction Affinity tagging has become a popular strategy for purifying recombinant proteins or functional domains in the postgenomic era. Fusing recombinant proteins with an amino acid sequence that selectively interacts with ligands on chromatographic matrices allows purification of virtually any protein without prior knowledge of its physical properties or physiological activities. This is...
متن کاملsynthetics of nife2o4 nanoparticles for recombinant his-tag protein purification
recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. differed methods for protein purification are categorized, based on cast, quality, easy work and side effect of protein. in this article, we are investigating his his-tag protein pu...
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ژورنال
عنوان ژورنال: Protein Science
سال: 2009
ISSN: 0961-8368,1469-896X
DOI: 10.1002/pro.112